Sequencing

Sequencing

Research Technique
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Research Technique

Determining the order of nucleotides of an DNA or RNA fragment. Sequencing may be applied to small and large amounts of nucleic acids, from a single gene to a whole genome.

EXAMPLES: Sanger Sequencing and Sequencing by Synthesis. Next generation sequencing techniques.

GSA deck

Graphic by David Orrdavidorogenic.com
DNA sequencing is the process of determining the precise order of nucleotides within a DNAmolecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and […] read more
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DNA sequencing is the process of determining the precise order of nucleotides within a DNAmolecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.

An example of the results of automated chain-termination DNA sequencing.

The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development offluorescence-based sequencing methods with a DNA sequencer,[1] DNA sequencing has become easier and orders of magnitude faster.[2]

(From: Wikipedia, June 2016)